Antibody humanization services

What is antibody humanization? Humanized antibodies are antibodies from non-human species whose amino acid sequences have been changed to improve their similarity to antibody variants produced naturally in humans. Before rodent antibodies can be used in clinical application is essential to humanize them to decrease their immunogenicity and increase their activity in the human immune system.

 

Antibody humanization is a key issue in therapy with antibodies

Monoclonal antibodies applications in therapy represents an increasing segments of the pharmaceutical industry. Hoever, there is a big problem because  monoclonal mouse antibodies are often immunogenic when they are used in humans since there are differences in the backbone amino acid sequences between mice and humans. Therefore, it is important to convert mice sequence into ones with human like sequences which can be done by humanization of monoclonal antibodies. The antibody humanization can decrease the antibody immunogenesity in humans therefore it can improve the success rate in therapy

 

Antibody humanization by CDR-Grafting

The six complementarity determining region (CDR) peptide loops in the roden antibodies are replaced into the human variable (V) regions.

This way to do antibody humanization is called CDR-grafting, and in some cases pure CDR-grafting can produce a humanized antibody with roughly the same antigen specificity and affinity as the original animal (usually mouse) antibody. The only choices required are which human V regions to graft the CDRs into and the isotype required to provide the desired biological function. The isotype choice is governed as for chimeric antibodies, but the choice of human V regions is more demanding.

 

Antbody humanization provides by Vrisko Ltd

Vrisko Limited offers services of antibody humanization including CDR grafting and screening of a human phage display antibody library.

Online Inquiry regarding these services.

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