Mouse Monoclonal Antibody Production

The most important requirements for successful monoclonal antibody generation include immunization strategies, antigen design, and screening technologies.  Our company  novel immunization procedures and careful mouse strain selection allows for production of monoclonal antibodies which are of the utmost affinity and specificity. This is because we take great care in designing the antigens for antibody production, especially with respect to synthesizing peptides, to generate a robust antibody that is able to recognize conformational changes of the native protein under the optimum number of conditions.
Mouse monoclonal antibody production is divided into different phases. Our price includes antibody production and guaranteed western blot results, which are not included in competitor’s services.

Mouse Monoclonal Antibody Production

The Custom monoclonal antibodies Basic package includes production of monoclonal antibodies by the hybridoma technology. In this package is not included recombinant protein expression, recombinant protein production or peptide synthesis used to humanized the mouse.

 

  1. Immunization (5 mice/antigen) and Sera Testing by ELISA.
  2. Fusion and Colony Screening.
  3. Subcloning, Colony Expansion and Freezing.
  4. Guaranteed western blot positive clones (2 clones)
  5. Antibody Purification from Ascites by Protein A/G (2 clones, 3mg each)

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