Long Taq DNA Polymerase

Long Taq DNA Polymerase is the mixture of two DNA polymerases designed for long PCR having the 3´ to 5´ proofreading exonuclease activity. Long Taq DNA Polymerase amplify long DNA fragments up to 20 kb for genomic DNA and up to 40 kb for viral DNA. Vrisko Limited provides Long Taq DNA Polymerase under the product name Vrisko LongTaq.

Vrisko LongTaq

APPLICATIONS:

• Standard and long PCR.
• PCR with difficult templates.

Figure 1. Amplification of 5,3 Kb target DNA using Vrisko LongTaq DNA Polymerase.
1- 2 – Vrisko LongTaq DNA Polymerase
M – Vrisko Star 1 Kb DNA Ladder Plus

Concentration: 5 unit/µl

Unit definition: One unit of Long Taq DNA Polymerase catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.

Source: Recombinant E. coli strain with cloned gene encoding Thermus aquaticus DNA polymerase.

Quality control: It was not detected non-specific nucleases.

Supplied with:
Vrisko 10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonident P40.
Vrisko 10x Taq Buffer with (NH₄)₂SO₄: 750 mM Tris-HCl (pH 8.8 at 25°C),
200 mM (NH₄)₂SO₄, 0.1% Tween 20.
Vrisko 25 mM MgCl₂

Storage conditions: 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Store at -20°C. Guaranteed stable for 12 months when properly stored.

Vrisko LongTaq is supplied for research use only.

Titan Taq DNA Polymerase

Titan Taq DNA Polymerase is highly processive, hot stable DNA polymerase. Titan Taq DNA Polymerase has 5’ to 3’ polymerisation-dependent exonuclease replacement activity but lacks 3’ to 5’ exonuclease activity. Titan Taq DNA Polymerase with its “extendase activity” permits  TA cloning. Vrisko Limited provides a Titan Taq DNA Polymerase under product name Vrisko Titan Taq.

Vrisko Titan Taq

Applications of  Vrisko Ltd´s Titan Taq DNA Polymerase

Titan Taq DNA Polymerase is useful for a wide range of PCR assays, primer extension, and TA cloning.

Figure 1. Amplification of 420 bp target DNA using Titan Taq DNA Polymerase.
M – Vrisko Star 100 bp DNA Ladder
1-3 – Vrisko Titan Taq DNA Polymerase

Concentration: 5 units/µl

Unit definition: One unit of  Titan Taq DNA Polymerase is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 min at 74ºC.

Source: Purified from an E. coli strain carrying an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.

Quality control: Titan Taq DNA Polymerase has not nicking-, priming- , exonucleases- or unspecific endonucleases activities. SDS/PAGE – 95 kD band, >98% pure. The activity and stability have been tested. The rate of error  per nucleotide per cycle is ~2.5 x 10^-5; the accuracy is ~ 4 x 10⁴. Estimated half life at 95°C is 1.5 hours.

Storage & Dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Supplied with:
10 x Reaction Buffer (Mg²⁺ free): 800 mM Tris-HCl pH 9.4 at 25°C, 200 mM (NH₄)₂SO₄, 0.2% w/v Tween-20.
25 mM MgCl₂

Shipping & Storage conditions: Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this reagent. However, routine storage at -20°C is strongly recommended.

Vrisko Titan Taq is supplied for research use only.

Pfu DNA Polymerase

Pfu DNA polymerase has 5´ to 3´ DNA polymerase activity and proofreading activity (3´ to 5´ exonuclease activity.  Pfu DNA Polymerase has much lower error rate than any commercially available DNA polymerase . Pfu DNA polymerase is superior than normal Taq DNA polymerase for amplifycations that require high-fidelity DNA synthesis. Our company provides pfu DNA polymerase under the product name Vrisko pfu.

Vrisko pfu

 Applications for our Vrisko Pfu DNA polymerase

• Vrisko Pfu DNA polymerase is up to ten times more accurate than normal DNA polymerase.
• Vrisko Pfu DNA polymerase produces blunt-ended amplification products to be used for cloning.
• Vrisko Pfu DNA polymerase remains active even after incubating 90 minutes at 95°C.

Concentration: Vrisko Pfu DNA polymerase contains 5 units/µl

Unit definition: One unit of Vrisko Pfu DNA polymerase catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.

Source: Recombinant E. coli strain with cloned gene encoding Pyrococcus furiosus DNA polymerase.

Quality control: it was not detected non-specific nucleases.

Supplied with:
Vrisko 10x Pfu Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 100 mM KCl,
100 mM (NH₄)₂SO₄, 1% Triton X-100, 1 mg/ml BSA
Vrisko 10x Pfu Buffer with MgSO₄: 200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM KCl,
100 mM (NH₄)₂SO₄, 1% Triton X-100, 1 mg/ml BSA, 25 mM MgSO₄
Vrisko 25 mM MgSO₄

Storage conditions: 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Store at -20°C. Guaranteed stable for 12 months when properly stored.

Vrisko pfu is supplied for research use only.

Titan Thermo DNA Polymerase

Titan Thermo DNA Polymerase is a highly processive hot stable  DNA polymerase usaful for MALDI-TOF mass spectrometry and other primer extension platforms. Titan Thermo DNA Polymerase has five prime to three prime  polymerisation-dependent exonuclease replacement activity ONLY and enhanced efficiency for incorporating unconventional nucleotides (labeled ddNTPs & acyclo nucleotides). Vrisko Ltd provides Titan Thermo DNA Polymerase under the product name Vrisko Titan Thermo.

Vrisko Titan Thermo

Applications:

• Primer extension.
• MALDI-TOF mass spectrometry.
• Mass-array.

Concentration: 5 units/µl

Unit definition: One unit of Vrisko Titan Thermo is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 min at 74°C.

Source: Purified from an E. coli strain carrying an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.

Quality control: Vrisko Titan Thermo DNA Polymerase is free of exonucleases-, endonucleases-, nicking-  and priming activities. The activity and stability of the enzyme was tested via thermocycling. Estimated half life at 95°C: >60 minutes. Error rate per nucleotide per cycle: ~8.0×10^-5.

Storage & Dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Supplied with:
10x Reaction Buffer Thermo: 500 mM Tris-HCl pH 9.5
100 mM MgCl₂

In some applications 0.5x Buffer final concentration is recommended.
0.5x Buffer final concentration is 25 mM Tris-HCl and 5 mM MgCl₂.

Shipping & Storage conditions: Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this reagent. However, routine storage at -20°C is strongly recommended.

Comments: Vrisko Titan Thermo is supplied for research use only.

Titan Hot Taq DNA Polymerase

 Titan Hot Taq DNA Polymerase is useful for hot start PCR. It is similar than Titan Taq DNA Polymerase but it has been modified. Titan Hot Taq DNA Polymerase is inactive at ambient temperatures without polymerase activity, but it is activated at 95-97°C during 15 minute.The advantage is that in this way you prevent the extension of non-specifically annealed oligonucleotides and primer-dimers formed at room temperature during PCR setup. Titan Hot Taq DNA Polymerase has 5’ to 3’ polymerisation-dependent exonuclease replacement activity but lacks 3’ to 5’ exonuclease activity. Titan Taq DNA Polymerase has “extendase activity” which allows TA cloning. Vrisko Ltd provides a Titan Hot Taq DNA Polymerase under the product name Vrisko Titan HotTaq.

Vrisko Titan HotTaq

Applications

• Hot Start PCR.
• Primer extension.
• TA cloning.

Figure 1. Amplification of ~500bp and ~600bp target DNA fragments using Vrisko Titan Hot Taq DNA Polymerase.
1-3 – Vrisko Titan HotTaq DNA Polymerase
M – Vrisko Star 1 Kb DNA Ladder

Concentration: 5 units/µl

Unit definition: Amount of Vrisko Titan HotTaq required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 min at 74°C.

Source: Purified from an E. coli strain carrying an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.

Quality control: The enzyme is free of nicking and priming-, exonucleases- and unspecific endonucleases activities. SDS/PAGE – 95 kD band, >98% pure. Activity and stability tested via thermocycling. The rate of error per nucleotide per cycle is ~2.5 x 10^-5; the accuracy is ~ 4 x 10⁴. Estimated half life at 95°C is 1.5 hours.

Storage & Dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Supplied with:
10x Reaction Buffer 1 (Mg²⁺, detergent free) Tris-HCl and (NH₄)₂SO₄
10x Reaction Buffer 2 (Mg²⁺ free) Tris-HCl, (NH₄)₂SO₄ and detergent
25 mM MgCl₂
10x Enhancer – Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution). Enhancer is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.

Shipping & Storage conditions: Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this reagent. However, routine storage at -20ºC is strongly recommended.

Comments: Vrisko Titan HotTaq is supplied for research use only.

Taq DNA polymerase

Taq DNA polymerase  is highly thermostable DNA polymerase that catalyzes synthesis of DNA from 5’ to 3’ .  The enzyme (Taq DNA polymerase) has not  exonuclease activity from 3’ to 5’ . Vrisko Ltd provides Taq DNA polymerase under the product name Vrisko Taq.

Vrisko Taq

Applications of our Taq DNA polymerase

• Vrisko Taq DNA polymerase can be used for general Polymerase chain reaction (PCR).
• Vrisko Taq DNA polymerase can be used for Primer extension.
• Vrisko Taq DNA polymerase can be used for DNA sequencing.

Figure 1. Amplification of 420 bp target DNA using Vrisko Taq DNA Polymerase.
M – Vrisko Star 100 bp DNA Ladder
1-3 – Vrisko Taq DNA plymerase

Concentration: Vrisko Taq DNA polymerase contains 5 units/µl

Unit definition: One unit of Vrisko Taq DNA polymerase catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.

Source: Vrisko Taq DNA polymerase is produced as a recombinant protein expressed in E. coli strain, from an expression vector carrying the gene encoding Thermus aquaticus DNA polymerase.

Quality control: non-specific nucleases have been detected.

Supplied with:

• Vrisko 10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonident P40.
• Vrisko 10x Taq Buffer with (NH₄)₂SO₄: 750 mM Tris-HCl (pH 8.8 at 25°C),
  200 mM (NH₄)₂SO₄, 0.1% Tween 20.
• Vrisko 25 mM MgCl₂

Storage conditions: 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Store at -20°C. Guaranteed stable for 12 months when properly stored.

Vrisko Taq is supplied for research use only.

Red Taq DNA Polymerase

Red Taq DNA Polymerase is Taq DNA polymerase that catalyzes the synthesis of DNA from 5´ to 3´ without having 3´ to 5´exonuclease activity. Red Taq DNA Polymerase  has high amplification yield and it is stable at high temperature.  Red Taq DNA Polymerase allows visual confirmation not needded the addition of loading dyes.  The amplified fragments can be loaded directly onto an agarose gel for electrophoresis. Our Red Taq DNA Polymerase has the product name Vrisko RedTaq.

Vrisko RedTaq

Applications of our RedTaq DNA Polymerase

• Vrisko RedTaq DNA Polymerase is suited for a wide range of PCR assays.
• Vrisko RedTaq DNA Polymerase is easy visualization of enzyme addition.
• Vrisko RedTaq DNA Polymerase allows visualization of complete reaction mixing.
• Vrisko RedTaq DNA Polymerase allows Direct loading of samples following amplification.

Figure 1. Amplification of 330 bp target DNA using Vrisko RedTaq DNA Polymerase.
1- 2 – Vrisko RedTaq DNA Polymerase
M – Vrisko Star 100 bp DNA Ladder

Concentration: Vrisko RedTaq DNA Polymerase contains 1 unit/µl

Unit definition: One unit of the Vrisko RedTaq DNA Polymerase catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.

Source: Vrisko RedTaq DNA Polymerase is produced by recombinant E. coli with have an expression vector carrying the gene encoding Thermus aquaticus DNA polymerase.

Quality control: non-specific nucleases were not detected.

Supplied with

• Vrisko 10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonident P40.
• Vrisko 10x Taq Buffer with (NH₄)₂SO₄: 750 mM Tris-HCl (pH 8.8 at 25°C),
  200 mM (NH₄)₂SO₄, 0.1% Tween 20.
• Vrisko 25 mM MgCl₂

Storage conditions: 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Store at -20°C. Guaranteed stable for 12 months when properly stored.

 Vrisko RedTaq is supplied for research use only.

Hot taq DNA polymerase

Hot taq DNA Polymerase is a hot start DNA polymerase that catalyzes the synthesis of DNA from 5´ to 3´.  A characteristic of Hot Taq DNA polymerase is that it has not  3´ to 5´ exonuclease activity.  Hot Taq DNA polymerase is a hot stable enzyme therfore it needs to be activated during 15 minutes at high temperature (95-97°C) before use it in PCR (Hot start PCR). Vrisko limited provides a Hot Taq DNA polymerase  for hot start PCR under the product name Vrisko HotTaq.

Vrisko HotTaq

 Applications of our Hot Taq DNA polymerase:

• Vrisko HotTaq is a Hot start DNA polymerase that can be used to setup PCR at room temperature.
• Vrisko HotTaq is a Hot Taq DNA polymerase that can be used for effective incorporation of modified nucleotides. 

Concentration: Vrisko Hot Taq DNA polymerase contains 10 units/µl

Unit definition: One unit of the Vrisko Hot Taq DNA polymerase catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.

Source : Vrisko Hot Taq DNA polymerase is produced in  E. coli expression system from an expression vector carrying the  gene encoding Thermus aquaticus DNA polymerase.

Quality control: Free of  non-specific nucleases. The activity and stability of the enzyme has been tested at 20, 30 and 40 cycles of PCR reaction at 95°C. It has been tested for the absence of human DNA contamination by PCR with Alu-specific primers.

Supplied with:

• Vrisko 10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonident P40.
• Vrisko 10x Taq Buffer with (NH₄)₂SO₄: 750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM (NH₄)₂SO₄, 0.1% Tween 20.
• Vrisko 25 mM MgCl₂

Storage conditions: 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Store at -20°C. Guaranteed stable for 12 months when properly stored.

Vrisko HotTaq is supplied for research use only.

Genomic DNA Isolation Kit

This is a quick and easy spin column method for the isolation of genomic DNA from blood. No phenol, chloroform, or other hazardous organic solutions are required. Proteins are degraded by Proteinase K, DNA is bound to the filter in the spin column, impurities are washed away, and the purified DNA is eluted from the binding matrix of the column. The isolated DNA is of excellent quality for PCR, restriction digestion, and other downstream applications. The kit contains all necessary tubes for the complete experiment in isolation of genomic DNA. This kit can also be used, without any modification, for isolating DNA from saliva, buccal cells, urine, semen, and cultured cells. Mini and Maxi preparation kits are available. Vrisko Ltd provides a Genomic DNA Isolation Kit under the product name Vrisko Genomic DNA Isolation Kit.